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PCR Boot Camp

Polymerase chain reaction (PCR) has been hailed as one of the most important advances in molecular biology and is an indispensable tool in biomedical research. The objective of this intensive 5-day hands-on PCR Boot Camp training is to impart transferrable skills on planning, setting-up, running and analysing PCR experiments.

Participants will be taken through principles of DNA replication, types and variants of PCR, applications, and PCR principles and workflows. They will learn how to design and synthesize primers, prepare samples, design PCR assays, set up software, report results, and apply PCR in DNA cloning for sequencing, functional gene analysis, disease diagnosis, paternity testing, and forensics. PCR Optimization and validation will also be taught. The course is right for any person who has an interest in starting to use PCR or current new users who want further training on Post-PCR Applications. 

PCR

At the end of this course, the participant will be able to:

  • Understand the Principles of DNA replication
  • Understand PCR principles and workflows
  • Understand other types of nucleic amplification such as isothermal methods
  • Design PCR and isothermal amplification primers and probes
  • Design PCR assays from sequence to primer and probe to actual experiments
  • Design and use CRISPR guide RNA (gRNA) in amplification-based detection of DNA
  • Extract DNA and RNA from a wide variety of samples
  • Run PCR reactions
  • Optimize and validate PCR reactions
  • Analyze real time PCR data
  • Perform post-PCR analysis including gel electrophoresis, cloning, and sequencing
  • Understand the diUnderstand the different applications of PCR
  • Course material including a comprehensive training manual
  • DNA Thermal cycler
  • Biosafety Cabinet
  • DNA Extraction Kit
  • Microcentrifuge
  • Adjustable pipettes (0.2-5ul, 5-10ul, 10-20ul, 10-100ul, 100-1000ul)
  • Gel electrophoresis apparatus
  • Vortex Mixer
  • 0.2 ml PCR tubes
  • TAE and TBE buffers
  • Agarose gel powder
  • PCR master mix
  • Primers
  • Pipette Filter Tips ((5ul, 10-20ul, 100ul, 1000ul)
  • Real time PCR machine
  • Analytical Balance
  • Dry Bath
  • NCBI Tools, Primer3 Plus
  • Nanodrop
    • Principles of DNA replication
    • PCR Types and Variants
    • Isothermal Amplification Methods
    • Restriction Endonucleases
    • CRISPR-CAS based DNA Detection
    • Primer and Probe Design
    • Sample Preparation: DNA/RNA Extraction, Nucleic acid quantification,
    • PCR principles and workflows: Initialization, Denaturation, Annealing, Extension/elongation, Final elongation, Final hold
    • Thermal Cycler components
    • PCR Setup and Cycling
    • Post-PCR Analysis
    • Result Reporting
    • PCR Optimization and validation
    • Applications of PCR: DNA cloning for sequencing, Functional gene analysis, Disease diagnosis, Paternity testing, Forensics
    The course is ideal for students and researchers working in the pharma, biotech, and medical industries. The following terms and conditions apply.
    • Minimum Entry Requirements: a bachelor’s degree in bioinformatics, genetics, molecular biology, biomedical science, microbiology or any related field
    • Email: [email protected] for fee inquiry or fill the contact form in the “Enroll” button
    • The fee is Kshs. 30,000. The registration fee covers tuition, laboratory consumables, course notes and refreshments. Please note that fees are payable in advance. Joining instructions will not be sent out until payment has been made in full.
    • Groups with a minimum of 5 people will enjoy a 10% discount and early bird registration (at least 2 weeks before the start) will be offered a 5% discount
    • There will be a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course. No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons.
    • KIBs is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Substitutions may, of course, be made at any time, providing you inform us in writing.
    Kindly fill the form details below to register for your course of interest.

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