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Nanopore Sequencing & Data Analysis Techniques

The Nanopore Sequencing & Data Analysis Techniques Course will equip the participant with skills on how to perform DNA sequencing using the Nanopore platform. Participants will also get hands-on skills on how to analyze Nanopore sequence data. The objective is to enhance the researchers’ capacity to conduct world class scientific research and boost their institution’s profile as a leading diagnostic and scientific center.
Nanopore Sequencing

At the end of the training, the participant will be able to:

  • Understand the science, methods, and applications of DNA and RNA sequencing
  • Identify the different nanopore sequencing devices, RNA kits, ligation sequencing kits, rapid sequencing kits, and low-input PCR kits and choose the ones best suited for their needs.
  • Identify the different barcoding kits and workflows
  • Prepare libraries for nanopore sequencing
  • Prime and load flow cells
  • Run sequencing using nanopore software such as MINKNOW
  • Understand nanopore sequencing data formats
  • Analyze sequencing data using EPI2ME tools
  • Assemble whole genomes from raw data and annotate it using common bioinformatics tools
  • Conduct BLAST analysis and other basic bioinformatics analyses


  • The MinION™ Mk1D
  • Windows 10/11 OS with 32 GB + RAM, NVIDIA RTX 4090 GPU, Intel I7 + (12-cores +) CPU and 2 TB SSD + storage
  • Internet connection
  • Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
  • PCR Thermocycler
  • Biosafety Cabinet
  • DNA Extraction Kit
  • Microcentrifuge
  • Nanodrop
  • Adjustable pipettes (0.2-5ul, 5-10ul, 10-20ul, 10-100ul, 100-1000ul)
  • Pipette Filter Tips ((5ul, 10-20ul, 100ul, 1000ul)
  • Vortex Mixer
  • 0.2 ml PCR tubes
  • PCR master mix
  • Software and Web Resources: MINKNOW, EPI2ME Tools, Porechop, Flye, NCBI Tools, Putty, WinScp
This is a 5-day intensive and hands-on training program covering the following topics:
  • Introduction to DNA/RNA sequencing
  • Nanopore sequencing principle, devices and kits
  • Barcoding kits and workflows
  • Library Preparation and Sequencing
  • Basic Bioinformatics
  • Whole Genome Assembly
  • Sequence Analysis
The course is ideal for students and researchers working in the pharma, biotech, and medical industries. The following terms and conditions apply.
  • Minimum Entry Requirements: a bachelor’s degree in bioinformatics, genetics, molecular biology, biomedical science, microbiology or any related field
  • Email: [email protected] for fee inquiry or fill the contact form in the “Enroll” button. The registration fee covers tuition, laboratory consumables, course notes and refreshments. Please note that fees are payable in advance. Joining instructions will not be sent out until payment has been made in full.
  • Groups with a minimum of 5 people will enjoy a 10% discount and early bird registration (at least 2 weeks before the start) will be offered a 5% discount
  • There will be a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course. No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons.
  • KIBs is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Substitutions may, of course, be made at any time, providing you inform us in writing.
Kindly fill the form details below to register for your course of interest.

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