Fluorescence Immunoassay – Laborex Fast Test Kit

Glycosylated Hemoglobin HbA1c immunoassay test uses an anti-human Hb monoclonal antibody conjugated with fluorescence latex and an anti-human HbA1C monoclonal antibody coated on the test line. After the sample has been applied on the test strip, the fluorescence latex-labelled anti-human Hb monoclonal antibody binds with the HbA1c and Hb in the sample proportional and forms marked antigen-antibody complex. The complex moves to the detection zone by capillary action. Then marked antigen-antibody complex is captured on the test line by the anti-human HbA1c monoclonal antibody. The fluorescence intensity of the test line increases in proportion to the amount of HbA1c in the sample.

The Laborex Immunoasy i100 is a rapid test that utilizes the HbA1c Cassette to identify HbAc based on immunofluorescence and calculates the value of the percentage of HbA1c in the sample.

Boronate Affinity Chromatography – A1C EZ 2.0 Glycohemoglobin Test Kit

This A1C EZ 2.0 Glycohemoglobin Analysis System utilizes a Boronate Affinity Chromatography method to quantitatively measure the level of Glycosylated Hemoglobin HbA1c in whole blood. A solid phase separation matrix is implemented as a membrane that has been chemically modified to contain both negatively charged groups and boronate groups. When the pH of the buffer flowing through the matrix is acidic, positively charged glycohemoglobin and non-glycohemoglobin bind to the negatively charged groups. When the pH is basic, the hemoglobin loses its charge and is released from the ionic binding. At basic pH, boronate binds not only cis-diols but also the glucose of glycohemoglobin holding the glycohemoglobin in the matrix. The analyzer uses an optical reflectance measurement technique to measure the membrane and quantify the hemoglobin bound in the matrix under each condition. The ratio of the glycohemoglobin to the total hemoglobin is calculated and reported as % HbA1c units.