Fragment Analysis

Fast and Accurate Fragment Analysis in Africa

Fragment Analysis is the gold standard in DNA sequencing and helps to validate Next Generation Sequencing (NGS) analysis.

Fragment analysis is a genetic analysis method comprising of a series of techniques in which DNA fragments are fluorescently labeled, separated by capillary electrophoresis (CE), and sized by comparison to an internal standard. In fragment analysis, PCR is used to produce fluorescently labeled DNA fragments using specific primers. Genetic analyzers can then size the fragments, quantify them, as well as genotype them. The resultant data can aid in the differentiation of alleles and identification of chimerism, homozygosity and heterozygosity, and sample mixtures.

Africa microsatelite analysis

Applications of Fragment Analysis

Microsatellite marker analysis – Short Tandem Repeats (STRs) and Variable Nucleotide Tandem Repeats (VNTRs) for Genetic Mapping, Linkage Analysis, and Inheritance Patterns. These are useful in human identification and determination of biological relationships such as DNA paternity testing Human Leucocyte Antigen (HLA) Typing to match patients and donors for cord blood or bone marrow transplants.

Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis.

Amplified Fragment Length Polymorphism (AFLP) Analysis.

Microsatellite Instability Analysis– useful in diagnosis of diseases such as hereditary non-polyposis colorectal cancer (HNPCC) (Lynch syndrome) and characterization of tumors and management of certain cancers such as colorectal, gastric, and endometrial cancer. MSI analysis accurately identifies tumors caused by defective MMR by comparing the number of nucleotide repeats in a panel of microsatellite markers in normal tissue with the number from tumor tissue from the same person.

Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis.

Authentication of cell lines – simple and inexpensive method of fingerprinting cell lines

Determination of CRISPR-Cas9 genome editing efficiency

Relative Fluorescent Quantitation: Loss of Heterozygosity (LOH), Aneuploidy Assays, and Large Chromosomal Deletion Detection

Analysis of chimerism

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Fragment Analysis Cost in Kenya

High Throughput (96-well) Plates50,000 Kshs
Individual Samples (genotyping)1,000 Kshs per reaction
MSI Analysis30,000 Kshs per sample
AFLP AnalysisInquire
Cell line authenticationInquire
Relative Fluorescent QuantitationInquire
Chimerism AnalysisInquire
Determination of CRISPR-Cas9 genome editing efficiencyInquire
T-RFLP AnalysisInquire



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Samples for Fragment Analysis

Sample Collection Instructions

Expert Bioinformatics Analysis

PhD-level bioinformatics analysis is bundled with the sequencing services and aids in the accurate identification of the following:

  • Single nucleotide polymorphisms (SNPs)
  • Structural variants (SVs) and multiple number variants (MNVs).
  • copy number variants (CNVs)
  • single nucleotide variants (SNVs)
  • Insertions and deletions (indels)
  • We also use cutting edge tools to explore, detect, quantify, and characterize epigenetic modifications.
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Fragment Analysis Features & Overviews

  • Simple Preparation

    Unlike sequencing, no DNA cleanup is necessary.

  • Simplified Data Analysis

    Data analysis is highly simplified by the genetic analysis software.

  • Multiplexing

    More than 20 loci can be evaluated in just a single reaction. This is possible because locus-specific primers are labelled with different colored dyes

  • Automated Workflows

    Ability to run thousands of DNA fragment samples in one day.

  • Short Turnaround Time

    24-48-hour turnaround from the time of sample receipt for most ready-to-run projects.

  • High Sensitivity

    Fragment analysis can accurately size fragments differing by only one base pair.


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