Fragment Analysis
Fast and Accurate Fragment Analysis in Africa
Fragment Analysis is the gold standard in DNA sequencing and helps to validate Next Generation Sequencing (NGS) analysis.
Fragment analysis is a genetic analysis method comprising of a series of techniques in which DNA fragments are fluorescently labeled, separated by capillary electrophoresis (CE), and sized by comparison to an internal standard. In fragment analysis, PCR is used to produce fluorescently labeled DNA fragments using specific primers. Genetic analyzers can then size the fragments, quantify them, as well as genotype them. The resultant data can aid in the differentiation of alleles and identification of chimerism, homozygosity and heterozygosity, and sample mixtures.
Applications of Fragment Analysis
Microsatellite marker analysis – Short Tandem Repeats (STRs) and Variable Nucleotide Tandem Repeats (VNTRs) for Genetic Mapping, Linkage Analysis, and Inheritance Patterns. These are useful in human identification and determination of biological relationships such as DNA paternity testing Human Leucocyte Antigen (HLA) Typing to match patients and donors for cord blood or bone marrow transplants.
Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis.
Amplified Fragment Length Polymorphism (AFLP) Analysis.
Microsatellite Instability Analysis– useful in diagnosis of diseases such as hereditary non-polyposis colorectal cancer (HNPCC) (Lynch syndrome) and characterization of tumors and management of certain cancers such as colorectal, gastric, and endometrial cancer. MSI analysis accurately identifies tumors caused by defective MMR by comparing the number of nucleotide repeats in a panel of microsatellite markers in normal tissue with the number from tumor tissue from the same person.
Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis.
Authentication of cell lines – simple and inexpensive method of fingerprinting cell lines
Determination of CRISPR-Cas9 genome editing efficiency
Relative Fluorescent Quantitation: Loss of Heterozygosity (LOH), Aneuploidy Assays, and Large Chromosomal Deletion Detection
Analysis of chimerism
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