Sanger Sequencing

Fast and Accurate Sanger Sequencing in Africa

Sanger sequencing is the gold standard in DNA sequencing and helps to validate Next Generation Sequencing (NGS) analysis.

Sanger is the preferred method for the identification of microbials, detection of SNPs, small construct/gene identification, and other applications which require a quick, cost effective solution. Sanger sequencing is also the preferred method to validate Next Generation Sequencing (NGS) analysis studies as well as regulatory submission studies. We provide a range of DNA sequencing services tailored to meet your needs and these include the Direct Colony Sequencing Service (DCSS), Single Pass DNA, full Sequence Analysis (FSA), and PCR and sequencing of genomic DNA targets. Sequence data and variant analysis results are publication quality.

Applications of Sanger Sequencing

Human Leucocyte Antigen (HLA) Typing to match patients and donors for cord blood or bone marrow transplants.

Mitochondrial Sequencing to determine one's maternal ancestry and for human identification purposes as well as in aging research and in the study of human diseases such as cancer and diabetes.

Low-level somatic variant detection in in material containing minimal amounts of DNA such as tumor FFPE samples.

Microbial genetics - to study microbes for purposes of disease control, gene cloning, research, and determination of drug resistance

To determine the accuracy of CRISPR and TALEN-mediated genome editing

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Sanger Sequencing Cost in Kenya

High Throughput (96-well) Plates50,000 Kshs
Individual Samples1,000 Kshs per reaction
Difficult Template500 Kshs added to the above cost
*Difficult templates are those that have hairpin structures, have GC content greater than 60% or high GC-content concentrated in a small region,have motifs causing band compressions, Bacterial Artificial Chromosomes (BACs) greater than 50kb, and contain long homopolymer stretches and di- and trinucleotide, direct, inverted and Alu repeats.

DNA Preparation Prices

For crude PCR products, bacterial colonies, pelleted cultures, and glycerol stocks
High Throughput (96-well Plates)
Plasmid Purification20,000 Kshs per plate
PCR and Cleanup20,000 Kshs per plate
PCR cleanup only500 Kshs added to the above cost
Individual Samples
Plasmid Purification500 Kshs per reaction
PCR and Cleanup500 Kshs per reaction
PCR cleanup only500 Kshs per reaction

Ready to Inject Plates

High Throughput (96-well) Plates10,000 Kshs
Individual Samples500 Kshs per reaction

Primer Synthesis

The cost of primer synthesis for supplied sequences is 100 Kshs per base and includes the following services:

Ordering of custom primer

Assessment of concentration and purity of primer

Diluting primer to appropriate concentration for reaction

Storage of primer on file and in facility freezer for 1 year

An additional 2,000 Kshs will be charged for primer design if you do not have the primer sequence.


Completed DNA Tests


Happy Clients


Customer Services



Success Rate

Samples for Sanger Sequencing

Sample Collection Instructions

Expert Bioinformatics Analysis

PhD-level bioinformatics analysis is bundled with the sequencing services and aids in the accurate identification of the following:

  • Single nucleotide polymorphisms (SNPs)
  • Structural variants (SVs) and multiple number variants (MNVs).
  • copy number variants (CNVs)
  • single nucleotide variants (SNVs)
  • Insertions and deletions (indels)
  • We also use cutting edge tools to explore, detect, quantify, and characterize epigenetic modifications.
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Whole Genome & Exome Sequencing Features & Overviews

  • Long reads

    long read sequencing resolves structural variants, repeat regions and phasing with long reads.

  • Direct Sequencing

    Direct RNA sequencing eliminates bias, allowing for a comprehensive assessment of epigenetic modifications

  • DNA Sample Collection Instructions

    Sample collection instructions

  • Microbial DNA

    Pathogens can be detected accurately and strains typed precisely.

  • Short Turnaround Time

    Results are available in 10 working days.

  • Confidentiality

    Client information is held in struct confidence and all data is returned to the client or discrded unlesss the client asks us to store it.


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