This hands-on training will equip participants with knowledge and skills on how to generate, manipulate, and analyze whole genome, whole exome, and targeted NGS data and discover germline SNPs, indels, SVs, and CNVs; somatic SNVs, SVs, and indels, and RNASeq SNPs and indels. The objective is to enhance the researchers’ capacity to conduct world class scientific research and boost their institution’s profile as a leading diagnostic and scientific centre..
The most robust GATK pipeline will be used
“Don#146;st let lack of lab equipment delay your project. Come and do it in our lab and finish your project on time.”
“ Besides high-tech equipment, we will also provide you with technical expertise. If you need help in data analysis and report writing, we will also be there to assist. ”
“Ever wanted to learn how to perform tests such as PCR but couldn#146;st? Vumbua is the place that allows you to operate lab equipment and gain hands-on skills.”
Using the most powerful software, you will gain the latest skills in NGS data analysis.
This training is ideal for people in biological, agricultural, pharma, and biotechnology industries who wish to gain skills on how to discover, design, and optimize drugs using in silico methods.The fee is Kshs. 85,000/- (850 USD, Inclusive of VAT 16%) for East African residents. Non-East African students pay 25% more on all charges.Fees covers tuition, training material, certificate, drinks and meals only. Vumbua members enjoy a 10% discount. Fees are payable strictly in advance (at least 4 working days before the starting date. Groups with a minimum of 5 people will enjoy a 10% discount and early bird registration (at least 2 weeks before the start) will be offered a 5% discount There will be a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course. No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons. KIBs is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Substitutions may, of course, be made at any time, providing you inform us in writing. Fee is payable by bankers cheque or MPESA to: Bioinformatics Institute of Kenya. A/C No. 0012342070001,Chase Bank, City Centre Branch, Nairobi, Kenya. The Paybill number for payments via Mpesa is 789190 and the account number is your name. Check the section below for online payments.
This is a 10-day intensive training program.
+ NGS Principles, methods, and technologies + NGS in Clinical Practice: Applications, Challenges and Promises.
+ Introduction to the Linux OS and basic commands + SSH, Telnet, FTP, WinScp, Putty, and Xming + Port forwarding and remote access + Accessing KenClust remotely
+ Obtaining datasets using wget and SRA toolkits. + Fasta file format + Fastq file format + SAM file format + BAM file format + Human reference genome (hg18 and hg19) + Indexing the reference genome + Fasta file index + Sequence directory
+ BWA commands + PE reads in interleaved and paired files + SE reads alignment + Alignment of short and long genomes + SAI to SAM to BAM + Sorting SAM files + Indexing SAM files + Duplicate marking + Local realignment + Base Quality Score Recalibration (BQSR)
+ HaplotypeCaller + Unified Genotyper + Annotation of SNPs and indels