{"id":859,"date":"2024-03-18T17:29:58","date_gmt":"2024-03-18T14:29:58","guid":{"rendered":"https:\/\/www.kibs.co.ke\/wp\/?page_id=859"},"modified":"2026-07-21T12:32:51","modified_gmt":"2026-07-21T09:32:51","slug":"pcr-boot-camp","status":"publish","type":"page","link":"https:\/\/www.kibs.co.ke\/research\/pcr-boot-camp\/","title":{"rendered":"PCR Boot Camp"},"content":{"rendered":"\n\n
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Polymerase chain reaction (PCR) has been hailed as one of the most important advances in molecular biology and is an indispensable tool in biomedical research. The objective of this intensive 5-day hands-on PCR Boot Camp training is to impart transferrable skills on planning, setting-up, running and analysing PCR experiments.<\/p>\n\n\n\n

Participants will be taken through principles of DNA replication, types and variants of PCR, applications, and PCR principles and workflows. They will learn how to design and synthesize primers, prepare samples, design PCR assays, set up software, report results, and apply PCR in DNA cloning for sequencing, functional gene analysis, disease diagnosis, paternity testing, and forensics. PCR Optimization and validation will also be taught. The course is right for any person who has an interest in starting to use PCR or current new users who want further training on Post-PCR Applications. <\/p>\n\n\n\n

\"PCR\"<\/figure>\n\n\n\n

At the end of this course, the participant will be able to:<\/p>\n\n\n\n\nUnderstand the Principles of DNA replication\n\n\n\nUnderstand PCR principles and workflows\n\n\n\nUnderstand other types of nucleic amplification such as isothermal methods\n\n\n\nDesign PCR and isothermal amplification primers and probes\n\n\n\nDesign PCR assays from sequence to primer and probe to actual experiments\n\n\n\nDesign and use CRISPR guide RNA (gRNA) in amplification-based detection of DNA\n\n\n\nExtract DNA and RNA from a wide variety of samples\n\n\n\nRun PCR reactions\n\n\n\nOptimize and validate PCR reactions\n\n\n\nAnalyze real time PCR data\n\n\n\nPerform post-PCR analysis including gel electrophoresis, cloning, and sequencing\n\n\n\nUnderstand the diUnderstand the different applications of PCR\n\n\n\n\n\n\n\n\n\nCourse material including a comprehensive training manual\n\n\n\nDNA Thermal cycler\n\n\n\nBiosafety Cabinet\n\n\n\nDNA Extraction Kit\n\n\n\nMicrocentrifuge\n\n\n\nAdjustable pipettes (0.2-5ul, 5-10ul, 10-20ul, 10-100ul, 100-1000ul)\n\n\n\nGel electrophoresis apparatus\n\n\n\nVortex Mixer\n\n\n\n0.2 ml PCR tubes\n\n\n\nTAE and TBE buffers\n\n\n\nAgarose gel powder\n\n\n\nPCR master mix\n\n\n\nPrimers\n\n\n\nPipette Filter Tips ((5ul, 10-20ul, 100ul, 1000ul)\n\n\n\nReal time PCR machine\n\n\n\nAnalytical Balance\n\n\n\nDry Bath\n\n\n\nNCBI Tools<\/a>, Primer3 Plus<\/a>\n\n\n\n\n\nNanodrop\n\n\n\n\n\nList Item\n\n\n\nList Item\n\n\n\n\n\n\n\n\n\nPrinciples of DNA replication\n\n\n\nPCR Types and Variants\n\n\n\nIsothermal Amplification Methods\n\n\n\nRestriction Endonucleases\n\n\n\nCRISPR-CAS based DNA Detection\n\n\n\n\n\nPrimer and Probe Design\n\n\n\nSample Preparation: DNA\/RNA Extraction, Nucleic acid quantification,\n\n\n\n\n\nPCR principles and workflows: Initialization, Denaturation, Annealing, Extension\/elongation, Final elongation, Final hold\n\n\n\nThermal Cycler components\n\n\n\n\n\nPCR Setup and Cycling\n\n\n\nPost-PCR Analysis\n\n\n\n\n\nResult Reporting\n\n\n\n\n\nPCR Optimization and validation\n\n\n\nApplications of PCR: DNA cloning for sequencing, Functional gene analysis, Disease diagnosis, Paternity testing, Forensics\n\n\n\n\n\n\n\n\nThe course is ideal for students and researchers working in the pharma, biotech, and medical industries. The following terms and conditions apply.\n\n\n\n\nMinimum Entry Requirements: a bachelor\u2019s degree in bioinformatics, genetics, molecular biology, biomedical science, microbiology or any related field\n\n\n\nEmail: training@kibs.co.ke for fee inquiry or fill the contact form in the \u201cEnroll\u201d button\n\n\n\n\n\nThe fee is Kshs. 30,000. The registration fee covers tuition, laboratory consumables, course notes and refreshments. Please note that fees are payable in advance. Joining instructions will not be sent out until payment has been made in full.\n\n\n\n\n\nGroups with a minimum of 5 people will enjoy a 10% discount and early bird registration (at least 2 weeks before the start) will be offered a 5% discount
\n\n\n\nThere will be a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course. No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons.\n\n\n\n\n\nKIBs is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Substitutions may, of course, be made at any time, providing you inform us in writing.\n\n\n\n\n\n\n\n\nKindly fill the form details below to register for your course of interest.\n\n\n\n