{"id":846,"date":"2024-03-18T13:30:08","date_gmt":"2024-03-18T10:30:08","guid":{"rendered":"https:\/\/www.kibs.co.ke\/wp\/?page_id=846"},"modified":"2026-07-21T12:45:21","modified_gmt":"2026-07-21T09:45:21","slug":"nanopore-sequencing-data-analysis-techniques","status":"publish","type":"page","link":"https:\/\/www.kibs.co.ke\/research\/nanopore-sequencing-data-analysis-techniques\/","title":{"rendered":"Nanopore Sequencing & Data Analysis Techniques"},"content":{"rendered":"\n\n
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\n\n\n\nThe Nanopore Sequencing & Data Analysis Techniques Course will equip the participant with skills on how to perform DNA sequencing using the Nanopore platform. Participants will also get hands-on skills on how to analyze Nanopore sequence data. The objective is to enhance the researchers\u2019 capacity to conduct world class scientific research and boost their institution\u2019s profile as a leading diagnostic and scientific center.\n\n\n\n
\"Nanopore<\/figure>\n\n\n\n

At the end of the training, the participant will be able to:<\/p>\n\n\n\n\nUnderstand the science, methods, and applications of DNA and RNA sequencing\n\n\n\nIdentify the different nanopore sequencing devices, RNA kits, ligation sequencing kits, rapid sequencing kits, and low-input PCR kits and choose the ones best suited for their needs.\n\n\n\nIdentify the different barcoding kits and workflows\n\n\n\nPrepare libraries for nanopore sequencing\n\n\n\nPrime and load flow cells\n\n\n\nRun sequencing using nanopore software such as MINKNOW\n\n\n\nUnderstand nanopore sequencing data formats\n\n\n\nAnalyze sequencing data using EPI2ME tools\n\n\n\nAssemble whole genomes from raw data and annotate it using common bioinformatics tools\n\n\n\nConduct BLAST analysis and other basic bioinformatics analyses\n\n\n\n\n\n\n\n\n\n\n\nThe MinION™ Mk1D\n\n\n\nWindows 10\/11 OS with 32 GB + RAM, NVIDIA RTX 4090 GPU, Intel I7 + (12-cores +) CPU and 2 TB SSD + storage\n\n\n\nInternet connection\n\n\n\nRapid Barcoding Kit 96 V14 (SQK-RBK114.96)\n\n\n\nPCR Thermocycler\n\n\n\nBiosafety Cabinet\n\n\n\nDNA Extraction Kit\n\n\n\nMicrocentrifuge\n\n\n\nNanodrop\n\n\n\nAdjustable pipettes (0.2-5ul, 5-10ul, 10-20ul, 10-100ul, 100-1000ul)\n\n\n\nPipette Filter Tips ((5ul, 10-20ul, 100ul, 1000ul)\n\n\n\nVortex Mixer\n\n\n\n0.2 ml PCR tubes\n\n\n\nPCR master mix\n\n\n\nSoftware and Web Resources: MINKNOW<\/a>, EPI2ME Tools<\/a>, Porechop<\/a>, Flye<\/a>, NCBI Tools<\/a>, Putty<\/a>, WinScp<\/a>\n\n\n\n\n\n\n\n\nThis is a 5-day intensive and hands-on training program covering the following topics:
\n\n\n\n\nIntroduction to DNA\/RNA sequencing\n\n\n\nNanopore sequencing principle, devices and kits\n\n\n\nBarcoding kits and workflows\n\n\n\nLibrary Preparation and Sequencing\n\n\n\nBasic Bioinformatics\n\n\n\nWhole Genome Assembly\n\n\n\nSequence Analysis\n\n\n\n\n\n\n\n\nThe course is ideal for students and researchers working in the pharma, biotech, and medical industries. The following terms and conditions apply.
\n\n\n\n\nMinimum Entry Requirements: a bachelor\u2019s degree in bioinformatics, genetics, molecular biology, biomedical science, microbiology or any related field\n\n\n\nEmail: training@kibs.co.ke for fee inquiry or fill the contact form in the \u201cEnroll\u201d button. The registration fee covers tuition, laboratory consumables, course notes and refreshments. Please note that fees are payable in advance. Joining instructions will not be sent out until payment has been made in full.\n\n\n\nGroups with a minimum of 5 people will enjoy a 10% discount and early bird registration (at least 2 weeks before the start) will be offered a 5% discount\n\n\n\nThere will be a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course. No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons.\n\n\n\nKIBs is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Substitutions may, of course, be made at any time, providing you inform us in writing.\n\n\n\n\n\n\n\n\nKindly fill the form details below to register for your course of interest.

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Course Registration Form<\/h2>\n\n
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Course Information<\/h3>\n\n
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